Journal: Frontiers in Immunology

Article Title: Homodimeric Minimal Factor H: In Vivo Tracking and Extended Dosing Studies in Factor H Deficient Mice

doi: 10.3389/fimmu.2021.752916

Figure Lengend Snippet: (A) Fluid phase assay of mouse C3b (mC3b) (350nM), human FI (75 nM) and 333nM of indicated cofactors (mouse full-length FH, mFLFH; mouse FH SCRs1-5, mFH1-5; human homodimeric minimal-FH, hHDM-FH; mouse HDM-FH, mHDM-FH and human FLFH, hFLFH). The mixture was incubated in solution at 37°C for 1 hour. C3b breakdown was analyzed by reducing SDS-PAGE and Coomassie staining followed by densitometry. A representative assay is shown, molecular weight standard is indicated to the left and C3 breakdown components noted to right of gel. The normalized density of C3b alpha prime chain remaining for each test reagent was calculated by dividing the density of the C3b alpha prime chain in the experimental lane by that of the ‘no cofactor’ control lane. The mean ± SD of remaining C3 alpha chain from 3 independent assays is noted in the graph. Any statistical difference between the efficacies of each cofactor in breaking down the C3b alpha prime chain was determined using Dunn’s multiple comparisons test. (B) The addition of increasing concentrations of mFH constructs prevented sheep erythrocyte lysis by mouse serum. Data shown is a combination of three independent experiments, transformed to log inhibitor (x-axis) normalised percentage protection (y-axis). Standard deviation for individual points with 95% CIs for sigmoidal curves is illustrated (C, D) . Evaluation of the binding properties of murine FH reagents to their physiological ligands. Doubly diluted concentration series of mHDM-FH (C) and mFLFH (D) , respectively, were flowed across a CM5 chip amine coupled with 930 RU of murine C3b at concentration between 10 and 0.019 µM. Inset shows a plot of RU against concentration, concentrations are also noted in the legend. Data shown is representative of 3 repeat experiments. One way ANOVA and Dunn’s multiple comparisons tests were applied – selected n.s. (non-significant) and the significant changes are shown. *p < 0.05.

Article Snippet: Briefly, 1μg mouse C3b (CompTech, Complement Technology, Inc., Tyler, Texas) was mixed with 100ng hFI (CompTech) and cofactors at 333nM: full-length hFH (hFLFH); mFH CCP 1-5 (mFH1-5); full-length mFH (mFLFH), hHDM- FH or mHDM-FH made up to a total volume of 15μl in PBS buffer before incubation at 37°C for 1 hour.

Techniques: Incubation, SDS Page, Staining, Molecular Weight, Control, Construct, Lysis, Transformation Assay, Standard Deviation, Binding Assay, Concentration Assay

Journal: Journal of Medical Internet Research

Article Title: Current State of Digital Biomarker Technologies for Real-Life, Home-Based Monitoring of Cognitive Function for Mild Cognitive Impairment to Mild Alzheimer Disease and Implications for Clinical Care: Systematic Review

doi: 10.2196/12785

Figure Lengend Snippet: Summary of 4 studies that included data derived from nondedicated ICT a solutions use, for example, secondary analysis of everyday computer use and pill box use (Group 4).

Article Snippet: Seelye (2015), United States [ ] , Mouse pointer movement variables were computed during routine home computer use using algorithms that identified and characterized mouse movements within each computer use session , Observational comparative study; 62 older adults living at home alone or who were the only computer user in the household; 1 week , 42 healthy (MMSE=28.8), 20 MCI (MMSE=27.3); mean age 87.9 and 87.5 years, respectively; 88% and 80% female, respectively , MCI was associated with making significantly fewer mouse moves and making mouse movements that were more variable, less efficient, and with longer pauses. Mouse movement significantly associated with several cognitive domains..

Techniques: Derivative Assay, Software